ABI OMICSS Guide 2021
  • Welcome
  • Week 1
    • Molecular Biology
      • Introduction to Biology
      • Macromolecules
      • DNA & RNA
      • Cell Division
    • Command-Line
      • Connecting to the Server
      • The Linux Command-Line For Beginners
      • The Bash Terminal
    • R Programming
      • Getting Started
      • The Basics
      • Data Types
    • Week 1 Exam
  • Week 2
    • Molecular Biology
      • DNA Proofreading And Repair
      • Telomeres and Telomerase
      • Genes
      • The One Gene, One Enzyme Hypothesis
      • Transcription
      • Translation
    • R Programming
      • Data Types (continuation)
      • Reading Data
      • Subsetting
      • Control Structures
      • Functions
      • Scoping Rules
    • Week 2 Exam
  • Week 3
    • Molecular Biology
      • tRNA and Ribosomes
      • Stages of Translation & Protein Targeting
      • Heredity
      • Probabilities In Genetics
    • R Programming
      • Loop Functions
      • Base Graphics
    • Statistics and R
      • The Basics
    • Week 3 Exam
  • Week 4
    • Molecular Biology
      • Interesting Cases of Genes
      • The Chromosomal Basis of Inheritance
      • Variation in Species
      • Phenotype plasticity
    • R Programming
      • Practice 2
      • Practice 3
    • Statistics and R
      • Random Variables and Probability Distributions
      • Central Limit Theorem
    • Week 4 Exam
  • Week 5
    • Statistics and R
      • Confidence Interval
      • Introduction to Inference
      • t-distribution and Comparing Means
      • Linear Models
    • Experimental Techniques
      • Polymerase Chain Reaction (PCR)
      • Gel Electrophoresis
      • Sanger Sequencing
      • NGS Sequencing
    • Week 5 Exam
  • Week 6
    • Statistics and R
      • Power
      • ANOVA
      • Covariance and Correlation
    • NGS
      • Basic Unix Commands
      • Sequences and Genomic Features
      • FastQC
      • Practice Exercises
    • Week 6 Exam
  • Week 7
    • Statistics and R
      • Monte Carlo Simulation
      • Exploratory Data Analysis
      • Linear Regression
    • NGS
      • BEDtools
      • Alignment and Sequence Variation
      • Integrated Genomics Viewer
    • Week 7 Exam
  • Week 8
    • NGS
      • Variant Calling With GATK
      • Practice 1
    • Week 8 Exam
  • Module Syllabi
  • Additional Resources
  • Conclusion
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  • What Is NGS?
  • Why Should I Learn About NGS?
  • What Are the Learning Outcomes of This Week?

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  1. Week 6

NGS

This module takes an estimated 3 hours to complete.

PreviousCovariance and CorrelationNextBasic Unix Commands

Last updated 3 years ago

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What Is NGS?

Next-generation sequencing (NGS) is a massively parallel sequencing technology that offers ultra-high throughput, scalability, and speed. The technology is used to determine the order of nucleotides in entire genomes or targeted regions of DNA or RNA. NGS has revolutionized the biological sciences, allowing labs to perform a wide variety of applications and study biological systems at a level never before possible. We briefly explored NGS in the .

Why Should I Learn About NGS?

Today's complex genomics questions demand a depth of information beyond the capacity of traditional DNA sequencing technologies. NGS has filled that gap and becomes an everyday tool to address these questions.

What Are the Learning Outcomes of This Week?

By the end of week 6, you should:

  • Be familiar with some of the basic Unix commands

  • Know about sequences and genomic features

  • Have fundamental knowledge of FastQC

  • Know about sequence recreation

  • Have good knowledge of SAMtools I and II

NGS Sequencing page of Experimental Techniques