> For the complete documentation index, see [llms.txt](https://docs.abi.am/abi-omicss-guide-2021/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://docs.abi.am/abi-omicss-guide-2021/week-5/experimental-techniques/sanger-sequencing.md). # Sanger Sequencing **DNA Sequencing** is a way to figure out the order of nucleotides along a strand of DNA. **Sanger sequencing** is based on the process of **DNA replication**. Scientists make copies of DNA strands. Then they observe which nucleotides are added. This way the sequence of nucleotides can be seen. To learn more about this topic, navigate through [this link](https://letstalkscience.ca/educational-resources/backgrounders/sanger-sequencing), and completely review the content on the page. Return here once you finish. ## Congratulations! If you made it here, then congratulations! You have successfully completed this section. Move to the next portion of the guide with the arrow buttons below. --- # Agent Instructions This documentation is published with GitBook. GitBook is the documentation platform designed so that both humans and AI agents can read, navigate, and reason over technical content effectively. Learn more at gitbook.com. ## Querying This Documentation If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question. Perform an HTTP GET request on the current page URL with the `ask` query parameter, and the optional `goal` query parameter: ``` GET https://docs.abi.am/abi-omicss-guide-2021/week-5/experimental-techniques/sanger-sequencing.md?ask=&goal= ``` `ask` is the immediate question: it should be specific, self-contained, and written in natural language. `goal` is optional and describes the broader end goal you are ultimately trying to accomplish on behalf of the user. GitBook uses it to tailor the answer towards what is most useful for that goal. The response will contain a direct answer to the question and relevant excerpts and sources from the documentation. Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.